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DOWNLOADS

LAB FILES

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PARTICLE TRAKCING
Brownian Motion

This program was written for a Brownian Motion Demo, in the Biophysics course (2019). The code was made in MatLab by Dr. Romina Munoz and allows the tracking of red particles when are recorded in blue/green background particles.

ENERGY LANDSCAPE
Brownian Dynamics

Program written for the Biophysics course. Code prepared in IgorPro (v7) enable to simulate a particle jumping between two state, changing the barrier and curvature of the potential.

POLYMER ELASTICITY AFM Data Analysis

Igor Procedure for the analysis of AFM-FX and AFM-FC experiments. The file is based on the software developed by Fernandez lab (Columbia Univ). The file was written on Igor v6.2, but also can be run on newer version (v7 and v8).

POLYMER ELASTICITY Multi-dLc Analysis

Igor Procedure for the analysis of AFM-FX including  with a new module for the fitting of multiple Lc of FX. The file is based on the software developed by Fernandez lab (Columbia Univ). The file was written on Igor v6.2, but also can be run on newer version (v7 and v8).

STEP COUNTER
MT Data Analysis

Software based on Igor (v7) for importing and fitting data collected on MT experiments. The panel allow to navigate through the trace recording the step size and the time of the event. Also allows to visualize consolidated step size distributions from the (un)folding events present on the recordings.

STEP COUNTER
MT Data Analysis

Software based on Igor (v7) for importing and fitting data collected on MT experiments. The panel allow to navigate through the trace recording the step size and the time of the event. Also allows to visualize consolidated step size distributions from the (un)folding events present on the recordings.

UNFOLD-DETECTOR
Kalman Filter

Software developed by Fernandez lab (Review of Scientific Instruments, 80,11, 2009, 113104) which capture automatically unfolding step on force extension recordings.

HALO-CHEMISTRY
Protocol for surfaces

The file contains step-by-step protocol for the preparation of Halo-ligand glass surfaces. These functionalized coversildes could be use either for AFM or MT experiments. Download the file for more details.

MT SOFTWARE
(C++)

Software written in C++ for controlling a ultra-fast Magnetic tweezers based. This software was originally developed by Fernandez Lab and considered a  voice-coil magnet positioner or electromagnet, and high speed camera (CMOS Ximea). This software allows data recording up to 1200 Hz.

MT SOFTWARE
(JACS 2016, 138)

Software written in IgorPro (v8) for controlling a Magnetic tweezers based. This software was originally developed by Fernandez Lab and considered a  voice-coil magnet positioner and high speed camera (CCD Pike). This software allows data recording up to 500 Hz.

THESIS ON TITIN ELASTICITY

Muscles are made of three main filaments: actin, myosin, and titin. Whereas the first two generate active contractile work, titin determinates the elasticity of the sarcomere. We are looking for highly motived student to join the lab on the exciting field of titin elasticity. Download the ad for more the details.

THESIS ON BACTERIAL ADHESION

Did you know that bacterias withstand hundreds of picoNewtons of force before detaching? We are looking student to join a new project that aims to understand the mechanisms involved on the bacterial adhesion. Download the ad with the details.

STUDENT'S THESIS

Click to Download or Website

Andrea San Martin
Biotech Engineer

Molecular Biology Platfomr for easy cloning and protein immobilization. This thesis was funded by ANID FONDECYT 11181075.

Tomas Hermosilla
Biotecnologist

Implementation of the first 3D printed Magnetic tweezers for the mechanical characterization of proteins. This thesis was funded by ANID FONDECYT 11181075.

Vicente Colarte
Biotechnologist

​Development of peptides to interfere with thee folding of Ig domians

This thesis was funded by ANID FONDECYT 11181075.

Pablo Berrios
Biotechnologist

Study of the elasticity of titin I10 domain under oxidative conditions. This thesis was funded by ANID FONDECYT 11181075.

Marcelo Jorquera
Biotechnologist

In silico characterization of AgI/II from S. mutans

This thesis was funded by ANID FONDECYT 1201064.

Nicolás Scherer
Biotechnologist

This thesis was funded by ANID FONDECYT 1201064.

Hector Vasquez
Biotechnologist

This thesis was supported by ANID FONDECYT 1201064.

Michelle Mendoza
Biotechnologist

This thesis was funded by ANID FONDECYT 11201064

Natalie Duchens
Biotechnologist

This thesis was funded by ANID FONDECYT 11201064

MULTIMEDIA

TRACKING A SINGLE BEAD

July, 2019

How molecules diffuse inside the cell? Using a small speaker, a Petri dish and two cell phones–for tracking and video recording–, we calculated the diffusion coefficient for the red particle (bead). We use a short MatLab routine to track the red bead along the video.  Video and MatLab script developed with the help of Romina Muñoz at SMAT-C USACH. Check files above.

GRABBING SINGLE MOLECULES

Jul 16, 2019

One of the Very first single molecule recording on Mjolnir after of the reassemble at the CGB Univ Mayor. Protein: I10 titin domain.

TRACKING A SINGLE BEAD

July 12, 2019

How molecules diffuse inside the cell? Using a small speaker, a Petri dish and two cell phones–for tracking and video recording–, we calculated the diffusion coefficient for the red particle (bead). We use a short MatLab routine to track the red bead along the video.  Video and MatLab script developed with the help of Romina Muñoz at SMAT-C USACH. Check files above.

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Isopeptide bond on Bacterial PILI

Aug 16, 2020

Streptococcus pyogenes is formidable colonizer of human epithelium. One of the reason is the presence of covalent bonds in the pili proteins that prevents the mechanical unfolding during the adhesion. This bond is the isopeptide bond, which is made between the side chain of a Lysine and Asparigine residue.

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+56 2 2328 1398

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Camino la Pirámide 5750, Huechuraba,

Santiago, Chile, 8580745

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