MECHANO-BIOLOGY GROUP

What We Do

Our group is interested in understand how mechanical forces modulate critical biological processes, from muscle elasticity to bacterial adhesion. Specifically, we aim to understand the mechanisms implemented within the cell in response to mechanical cues, describing the genes involved and how the proteins coded by those genes are adapted in nature.

In 2020 we established a mechanical assay that allowed us to characterize the giant protein of striated muscle, titin (Nature Comm 11; 2060). These experiments will help us to contribute to determining the emerging role that this gene and protein play in the myocyte.

Furthermore, we have implemented several experiments that aim to determine how a gene family highly conserved in the Group A Streptococcus—adhesin gene—, is involved in the adhesion to human epithelia. We analyze the evolutive differences found in the sequences, and how these changes are translated to protein structure and modify the adhesion mechanisms (Methods Mol Biol. 2020; 2136:347-364).

Currently we are working to develop new strategies for interfering with the folding and formation of bacterial adhesins. We are combing computational tools, protein engineering and single molecule mechanics to probe our anti-adhesive peptides.

Publications

Research

A LITTLE ABOUT THE LAB

We started the lab on early 2019, focusing our efforts to set up a research line on the emerging field of the mechano-biology, the interface between biology, chemistry, and physics. Our motivation is to understand how mechanicial processes modulate biological responses. We use a combination of molecular biology, protein engineering, computational tools, and biophysics aiming to determine how force become one of the most important signals in the cell.

We are located at Universidad Mayor in the Center for Genomics and Bioinformatics (CGB), Laboratory of Microbe Genomics.Thanks to our collaborators at Fernádez lab (Columbia Univ) we assemblied of our first single-molecule instrument also know as Mjölnir, the Thor's hammer. The microscope is a modified atomic force microscope designed for mechanical pulling at the single cell and molecule level.

In 2022, we built the very first 3D printed based Magnetic-Tweezers instrument, which is fully available in our Github. Inspired in the designed of Julio Fernández and our collaborator Rafael Tapia Rojo, Stormbreaker or Thor's Axe, we are able to apply calibrate forces on single molecules from <1 pN to 100 pN. Currently we are working in a third setup, which is an automatized version of our MT-force spectrometer equipped with an a motorized stage and particle recognition.

Check it out our research projects!